Using Analytical Ultracentrifugation applied to monoclonal antibodies
Analytical ultracentrifugation (AUC) is a powerful technique employed for the characterization of antibodies, offering detailed insights into their size, anisotropy, and interactions. This method involves centrifuging samples to generate sedimentation profiles, which can be analyzed to determine molecular weights, oligomeric states, and conformational changes. AUC is particularly advantageous for antibody characterization, as it operates in solution, preserving the native state of the molecules and allowing for the study of their behavior in physiologically relevant conditions. The technique provides high-resolution data critical for understanding antibody stability, aggregation, and complex formation, making it an invaluable tool in the development and quality control of therapeutic antibodies.
